Table 2 Comparison of transformation using different explants and vectors in the R108 ecotype
Cultivar | Material/method used for transformation | Vectors | Number of calli | Resistant explants | Regenerated plants from initial explants |
|---|---|---|---|---|---|
R108 crn mutant | Shoot bisections protocol 1 | p35S:CRN:YFP | 120 | 61 (50%) | 25 (21%) |
R108 crn mutant | Root segments protocol 2A | p35S:CRN:YFP | 80 | 36 (45%) | 7 (9%) |
R108 | Shoot bisections protocol1 | pTCSN1 | 108 | 55 (51%) | 16 (15%) |
R108 | Root segments protocol 2B | pTCSN1 | 42 | 17 (40%) | 8 (19%) |