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Fig. 3 | Plant Methods

Fig. 3

From: Comparison of efficiency and time to regeneration of Agrobacterium-mediated transformation methods in Medicago truncatula

Fig. 3

Steps in Tissue Culture Protocol 1. a Initial cut on 4 day old seedling. b Bisected shoot starting material (cotyledons cut off). c Shoot bisection starting material. d Co-cultivation with filter paper on media. e Inserting half of explant into agar so the meristem contacts the PPT medium. f Untransformed controls and g Explants transformed with construct after 2 rounds of selection of 15 days each. h Transfer to shoot development media. I, J Shoots arising from h. k Intact plants in magenta box or l Test tube. m, n Regenerated plants ready for transfer into soil

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