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Fig. 2 | Plant Methods

Fig. 2

From: Comparison of efficiency and time to regeneration of Agrobacterium-mediated transformation methods in Medicago truncatula

Fig. 2

Confirmation of the transformations. a lanes 1-11 are PCR of a fragment of the bar gene (expected size 554 bp) using DNA from 11 individual T0 transformation plants of pTCSN1::GFP-ER as template. The bar gene fragment was not detected in wild type plants. b Lanes 1-6 are PCR amplification of a CRN gene fragment (expected size 526 bp) using DNA from 6 individual T0 transformation plants of crn as template. Lane 7 template uses DNA from an untransformed crn mutant plant. c Green fluorescence observed in the roots of T0 transformed plants of pTCSN1::GFP-ER before treatment. d Green fluorescence observed in in the roots of T0 transformed plants of pTCSN1::GFP-ER after 24 h treatment of 15 μM BAP

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