Table 2 Distribution of Cas9:sgRNA induced mutations in the HvCKX1 gene in T0 plants and their segregation in T1 progeny
T0 plant ID | No. of mutant and wta clones in a T0 plantb | Segregation of mutations in T1 lines [No. of wt, ht, and hm plants] | Segregation of T-DNA in T1 lines | No. of mutated, T-DNA free plants in T1 lines | |||
|---|---|---|---|---|---|---|---|
Mutant | wtb | wt | ht | hm | PCR(+):PCR(−) | ||
22 | 10 | 0 | 0 | 5 | 5 | 7:3 | 3 |
27 | 10 | 0 | 7 | 2 | 1 | 2:8 | 3 |
28 | 10 | 0 | 10 | 0 | 0 | 8:2 | 0 |
29 | 9 | 1 | 3 | 5 | 2 | 7:3 | 3 |
30 | 10 | 0 | 7 | 3 | 0 | 8:2 | 0 |
32 | 10 | 0 | 6 | 4 | 0 | 8:2 | 1 |
33 | 10 | 0 | 8 | 2 | 0 | 3:7 | 0 |
34 | 10 | 0 | 0 | 1 | 9 | 10:0 | 0 |
37 | 8 | 2 | 4 | 6 | 0 | 4:6 | 2 |
38 | 10 | 0 | 0 | 8 | 2 | 9:1 | 1 |
41 | 9 | 1 | 6 | 4 | 0 | 5:5 | 2 |
42 | 10 | 0 | 5 | 5 | 0 | 7:3 | 0 |
44 | 8 | 2 | 1 | 8 | 1 | 9:1 | 0 |
45 | 10 | 0 | 6 | 4 | 0 | 9:1 | 0 |
47 | 7 | 3 | 6 | 4 | 0 | 9:1 | 0 |
54 | 10 | 0 | 6 | 3 | 1 | 6:4 | 1 |
57 | 10 | 0 | 2 | 5 | 3 | 7:3 | 2 |
63 | 10 | 0 | 5 | 3 | 2 | 7:3 | 1 |
67 | 10 | 0 | 6 | 4 | 0 | 8:2 | 1 |
70 | 10 | 0 | 7 | 3 | 0 | 7:3 | 0 |
78 | 10 | 0 | 6 | 2 | 2 | 8:2 | 0 |
80 | 10 | 0 | 6 | 4 | 0 | 8:2 | 0 |
Total number of T1 plants: | 107 | 85 | 28 | 156:64 | 20 | ||