Fig. 2
Quality control of ATAC-seq libraries. a Chromatin accessibility at four genomic loci (90 bp) measured by qPCR. Left, the amplification of each region was normalized to genomic DNA at the same locus. Primer sets 1 and 2 amplify open (accessible) regions, whereas primer sets 3 and 4 amplify closed (inaccessible) regions. Right, quality control score calculated as the ratio of the average of the two open versus closed sites. NS—an example of a library with nonspecific digestion. Error bars indicate standard deviation of three technical repeats. ***p < 0.001, ANOVA and Tukey’s test. For NS library, p = 0.95 b Genomic browser view of the regions amplified in (a) after sequencing (aligned to TAIR10)