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Table 2 Definitive reference genes under multiple treatments and tissues in H. brevisubulatum

From: Reference genes identification for normalization of qPCR under multiple stresses in Hordeum brevisubulatum

Treatment Optimal single reference gene Optimal reference gene combination
NaCl ADP ADP UBI
PEG6000 EF- EF-1α UBI
Mannitol UBI EF-1α UBI
ABA UBI UBI 18SrRNA-1 18SrRNA-3
GA3 EF- EF-1α 18SrRNA-3
Ethylene EF- EF-1α TUBα
Cold 18SrRNA-3 18SrRNA-3 UBI
Heat EF- EF-1α 18SrRNA-1 ADP
Tissues TUBα TUBα UBI TUBβ6