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Table 2 Definitive reference genes under multiple treatments and tissues in H. brevisubulatum

From: Reference genes identification for normalization of qPCR under multiple stresses in Hordeum brevisubulatum

Treatment

Optimal single reference gene

Optimal reference gene combination

NaCl

ADP

ADP UBI

PEG6000

EF-1α

EF-1α UBI

Mannitol

UBI

EF-1α UBI

ABA

UBI

UBI 18SrRNA-1 18SrRNA-3

GA3

EF-1α

EF-1α 18SrRNA-3

Ethylene

EF-1α

EF-1α TUBα

Cold

18SrRNA-3

18SrRNA-3 UBI

Heat

EF-1α

EF-1α 18SrRNA-1 ADP

Tissues

TUBα

TUBα UBI TUBβ6