Fig. 2

Microscopic observations of pollen semi-sterility caused by S₁ and S₁₉ in the heterozygous state. a–e Mature pollen stained with I₂-KI solution in a S ^g ₁ /S ^s ₁ heterozygotes, b S ^g ₁ homozygotes, c O. sativa ‘Taichung65’ (S ^s ₁ and S ^s ₁₉ homozygotes), d S ^g ₁₉ /S ^s ₁₉ heterozygotes, and e S ^g ₁₉ homozygotes. Bar, 50 µm. f Pollen fertility exhibited by each genotype of S₁ and S₁₉ loci. Error bars indicate standard errors. Different lowercase letters between bars indicate significant differences at the 5% level (Welch’s t test corrected by Bonferroni’s method). g Aberrant microspores (indicated by the arrowhead) of S ^g ₁ /S ^s ₁ heterozygotes stained with acetocarmine at the late uninucleate stage. Bar, 10 µm