Fig. 1
From: Investigating the microstructure of plant leaves in 3D with lab-based X-ray computed tomography
Step-by-step stages of the microCT workflow. a Excising the leaf discs, b sample mounting schematic, c image of mounted sample, d X-ray CT scanning and e–i image analysis workflow for extracting intercellular airspace from plant leaf scans, illustrated using both side-on (ZY orientation, top row) and top-down (XY orientation, palisade mesophyll layer, bottom row) views of an Arabidopsis thaliana leaf selection (400 × 400 pixels). Resolution = 2.75 µm. Scale bars = 0.4 mm