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Fig. 1 | Plant Methods

Fig. 1

From: A protocol combining multiphoton microscopy and propidium iodide for deep 3D root meristem imaging in rice: application for the screening and identification of tissue-specific enhancer trap lines

Fig. 1

Effect of the Ca2+ concentration and the stage of development on the fluorescence of propidium iodide. Root tips of Oryza sativa cv Nipponbare rice seedlings (3, 6 and 8 days after germination) were incubated for 10 min in a 10 μM propidium iodide Merck H2O solution, with 0–100 μM CaCl2. The root tips were then rinsed twice in Merck H2O, and a median view (150 μm of the root tip surface) was always imaged with the same device settings (Wavelength: 1097 nm, Laser Power laser: 50, gain: 600, image resolution 1024 × 1024, speed 7, and average 8)

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Plant Methods

ISSN: 1746-4811