Fig. 4
From: Cryo-laser scanning confocal microscopy of diffusible plant compounds
Cryo-observation of apple a, c, e and grape b, d, f tissues infused by Fluo-3 (calcium), PBFI (potassium), DPBA (flavonoid) dyes and cryo-fixed in cooled isopentane. Fluorescence observations in fresh apple fruit (g, i, k) and grape berry (h, j, l) sections directly stained by Fluo-3, PBFI and DPBA. C: Cuticle, CW: Cell Wall. Scale bar: 50 µm for calcium probe and 100 µm for potassium and flavonoid probes. Laser excitation and gain are indicated to stress the difference in fluorescence intensity recovered between fresh and frozen samples. The applied fluorescence intensity of the cuticle is due in part to autofluorescence related to the presence of phenolic compounds