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Table 7 Comparison between the HT-SuperSAGE expression libraries and qPCR data in cowpea roots under abiotic stress treatments

From: Cowpea and abiotic stresses: identification of reference genes for transcriptional profiling by qPCR

Gene Root dehydration Salt stress (NaCl, 100 mM)
HT-SS assay† qPCR assay* HT-SS assay† qPCR assay*
(Pingo de Ouro) TOL VGEǂ (Santo Inácio) SEN VGEǂ (Pitiúba) TOL VGEǂ (BR14-Mulato) SEN VGEǂ
TOL SEN 25′ 75′ 150′ 25′ 75′ 150′ TOL SEN 30′ 60′ 90′ 30′ 60′ 90′
VuChiB UR ns ns ns ns No UR ns ns Yes ns UR ns ns UR Yes DR DR ns No
VuLTP UR DR ns ns UR Yes ns ns ns No UR DR UR UR UR Yes ns ns ns No
VuCHI UR UR ns UR UR Yes UR UR UR Yes UR ns UR UR UR Yes UR UR UR No
VuCHS UR ns UR UR UR Yes UR UR UR No ns UR UR UR UR No UR UR ns Yes
  1. HT-SS (HT-SuperSAGE); Vu (Vigna unguiculata); VuCHiB (Chitinase B); VuLTP (Lipid transfer protein); VuCHS (Chalcone synthase); VuCHI (Chalcone isomerase)
  2. † and * p < 0.05. TOL (tolerant accession); SEN (sensitive accession); UR (up-regulated); DR (down-regulated); ns (not significant at p < 0.05); 25′, 75′ and 150′ (minutes under root dehydration); 30′, 60′ and 90′ (minutes under salt stress), VGE (Validation of Gene Expression); ǂ between HT-SuperSAGE and qPCR data