Table 7 Comparison between the HT-SuperSAGE expression libraries and qPCR data in cowpea roots under abiotic stress treatments
From: Cowpea and abiotic stresses: identification of reference genes for transcriptional profiling by qPCR
Gene | Root dehydration | Salt stress (NaCl, 100 mM) | ||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
HT-SS assay† | qPCR assay* | HT-SS assay† | qPCR assay* | |||||||||||||||||
(Pingo de Ouro) TOL | VGEǂ | (Santo Inácio) SEN | VGEǂ | (Pitiúba) TOL | VGEǂ | (BR14-Mulato) SEN | VGEǂ | |||||||||||||
TOL | SEN | 25′ | 75′ | 150′ | 25′ | 75′ | 150′ | TOL | SEN | 30′ | 60′ | 90′ | 30′ | 60′ | 90′ | |||||
VuChiB | UR | ns | ns | ns | ns | No | UR | ns | ns | Yes | ns | UR | ns | ns | UR | Yes | DR | DR | ns | No |
VuLTP | UR | DR | ns | ns | UR | Yes | ns | ns | ns | No | UR | DR | UR | UR | UR | Yes | ns | ns | ns | No |
VuCHI | UR | UR | ns | UR | UR | Yes | UR | UR | UR | Yes | UR | ns | UR | UR | UR | Yes | UR | UR | UR | No |
VuCHS | UR | ns | UR | UR | UR | Yes | UR | UR | UR | No | ns | UR | UR | UR | UR | No | UR | UR | ns | Yes |