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Table 4 Characterization of cowpea qPCR reactions, indicating the category of the primer pairs used, amplified CRG or TT name, source (reference), efficiency (%) and sensitivity (y-intercept)

From: Cowpea and abiotic stresses: identification of reference genes for transcriptional profiling by qPCR

Category Name Reference Slope (−) Efficiency (%) R2 y-intercept
CRG VuACT CpFGC 3.18 106.28 − 0.989 35.09
CRG β-TUB [25] 3.25 103.09 − 0.993 35.34
CRG EF1-α [26] 3.38 97.63 − 0.995 33.12
CRG FBOX [27] 3.28 101.78 − 0.982 35.77
CRG UE21D [27] 3.30 100.92 − 0.995 34.63
CRG VuUBQ10 CpFGC 3.40 96.84 − 0.997 38.01
CRG UNK [27] 3.35 98.84 − 0.989 35.17
TT VuChiB CpFGC 3.40 96.84 − 0.996 38.03
TT VuLTP CpFGC 3.38 97.24 − 0.995 35.56
TT VuCHI CpFGC 3.43 95.68 − 0.996 37.01
TT VuCHS CpFGC 3.31 100.50 − 0.996 33.69
  1. CRG (Candidate Reference Gene); TT (Target Transcript); Cowpea Functional Genome Consortium (CpFGC); Vu (Vigna unguiculata); β-TUB (beta-tubulin); EF1-α (elongation factor 1-alfa); VuACT (actin); UE21D (ubiquitin-conjugating enzyme E2 variant 1D); UNK (Phaseolus vulgaris unknown gene); FBOX (F-box protein); VuUBQ10 (polyubiquitin 10); VuCHiB (chitinase B); VuLTP (lipid transfer protein); VuCHI (chalcone isomerase); VuCHS (chalcone synthase); R2 (Coefficient of Determination)