Cowpea and abiotic stresses: identification of reference genes for transcriptional profiling by qPCR

Table 4 Characterization of cowpea qPCR reactions, indicating the category of the primer pairs used, amplified CRG or TT name, source (reference), efficiency (%) and sensitivity (y-intercept)

Category	Name	Reference	Slope (−)	Efficiency (%)	R²	y-intercept
CRG	VuACT	CpFGC	3.18	106.28	− 0.989	35.09
CRG	β-TUB	[25]	3.25	103.09	− 0.993	35.34
CRG	EF1-α	[26]	3.38	97.63	− 0.995	33.12
CRG	FBOX	[27]	3.28	101.78	− 0.982	35.77
CRG	UE21D	[27]	3.30	100.92	− 0.995	34.63
CRG	VuUBQ10	CpFGC	3.40	96.84	− 0.997	38.01
CRG	UNK	[27]	3.35	98.84	− 0.989	35.17
TT	VuChiB	CpFGC	3.40	96.84	− 0.996	38.03
TT	VuLTP	CpFGC	3.38	97.24	− 0.995	35.56
TT	VuCHI	CpFGC	3.43	95.68	− 0.996	37.01
TT	VuCHS	CpFGC	3.31	100.50	− 0.996	33.69

CRG (Candidate Reference Gene); TT (Target Transcript); Cowpea Functional Genome Consortium (CpFGC); Vu (Vigna unguiculata); β-TUB (beta-tubulin); EF1-α (elongation factor 1-alfa); VuACT (actin); UE21D (ubiquitin-conjugating enzyme E2 variant 1D); UNK (Phaseolus vulgaris unknown gene); FBOX (F-box protein); VuUBQ10 (polyubiquitin 10); VuCHiB (chitinase B); VuLTP (lipid transfer protein); VuCHI (chalcone isomerase); VuCHS (chalcone synthase); R² (Coefficient of Determination)

ISSN: 1746-4811