Cowpea and abiotic stresses: identification of reference genes for transcriptional profiling by qPCR

Table 3 Transcriptional modulation of selected cowpea targets transcripts in the accessions and treatments analyzed

Gene	Unitag name	Root dehydration				Salt stress (NaCl, 100 mM)
		Tolerant accession (Pingo de Ouro)		Sensitive accession (Santo Inácio)		Tolerant accession (Pitiúba)		Sensitive accession (BR14-Mulato)
		FC	Reg. (*)	FC	Reg. (*)	FC	Reg. (*)	FC	Reg. (*)
VuChiB	Cp1020	5.10	UR	− 1.09	ns	1.20	ns	4.40	UR
VuLTP	Cp1050	11.21	UR	− 1.94	DR	2.80	UR	− 7.90	DR
VuCHI	Cp1131	5.65	UR	1.15	ns	1.20	ns	9.50	UR
VuCHS	Cp2022	47.70	UR	13.70	UR	2.20	UR	1.40	ns

Reg. (gene regulation); FC [Fold change: measure describing how much a quantity changes going from an initial (control) to a final value (treatment)]; UR: (up-regulated); DR: (down-regulated); ns (not significant at the level of p ≤ 0.05). Vu (Vigna unguiculata); VuCHiB (chitinase B); VuLTP (lipid transfer protein); VuCHI (chalcone isomerase); VuCHS (chalcone synthase)
*Gene regulation at the level of p ≤ 0.05

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