Fig. 5
From: Functional intron-derived miRNAs and host-gene expression in plants
Activity of ffgLUCaimiR-PHYB in transient assays (a) and stable transformed plants (b, c). a Relative LUC activity of ffgLUC and ffgLUCaimiR-PHYB at 4 days post-agro-infiltration of Nicotiana benthamiana leaves (n = five leaves per treatment). b Relative LUC activity in eight independentstable transformants of Arabidopsis thaliana expressing either ff-gLUC or ffgLUCaimiR-PHYB. c Average relative expression level of AthPHYB in five ff-gLUC and ffgLUCaimiR-PHYB plants (reference gene AthActin1, expression of AthPHYB normalized to that in one ffgLUC plant). d Image of LUC activity in representative transgenic plants expressing ff-gLUC (left) or ffgLUCaimiR-PHYB (right). e Detection of mature amiR-PHYB by stem-loop RT PCR analysis in small RNA isolated from ffgLUCaimiR-PHYB plants but not in small RNA isolated from control ffgLUC plants