Fig. 4
From: Functional intron-derived miRNAs and host-gene expression in plants
Evaluation of silencing in cis- and trans in transient assays (a) and stable transformed plants (b, c). a Left: evaluation silencing in-cis transient assay: LUC activity of ffgLUC and ffgLUCaimiR-LUC at 4 days post-agro-infiltration in Nicotiana benthamiana transient assay (n = five leaves per treatment). Right: evaluation silencing in-trans transient assay: LUC activity in Nicotiana benthamiana transient assay of ffcLUC co-infiltrated with empty vector and ffcLUC co-infiltrated with amiR-LUC at 4 days post-agro-infiltration (n = five leaves per treatment). Quantification of the DsRED gene expression was used to confirm similar transformation efficiencies in the agro-infiltration with ffgLUC and ffgLUCaimiR-LUC (Additional file 1: Fig. S3). b Evaluation of silencing in-cis: Relative LUC activity in sixteen independent T1 generation of ffgLUC and ffgLUCaimiR-LUC plants. “A” indicates average LUC activity in set of transgenic plants. Significant differences between samples (***) is based on standard error (student’s t test, P < 0.01). c Evaluation of silencing in-trans: Relative LUC activity in homozygous ffcLUC line and T1 of same ffcLUC line transformed with 2×35S:amiR-LUC. Significant differences between samples (***) is based on standard error (student’s t test, P < 0.01)