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Fig. 1 | Plant Methods

Fig. 1

From: Functional intron-derived miRNAs and host-gene expression in plants

Fig. 1

Structure of aimiRNA expression constructs. 1 The Firefly Luciferase gene with intron 2×35S:ffgLUC. 2 aimiRNA gene with miR319a in ffgLUC intron with small deletion: 2×35S:ffgLUC aimiR-319adel . 3 aimiRNA gene with miR319a in ffgLUC intron without deletion: 2×35S:ffgLUCaimiR-319a. 4 aimiRNA gene targeting AthPHYB: 2×35S:ffgLUCaimiR-PHYB. 5 aimiRNA gene targeting ffLUC: 2×35S:ffgLUCaimiR-LUC. “a” indicates the intron branch point site, “gu” indicates the 5′-splice site and “ag” indicates the 3′-splice site. Exchange of 21 bp target sequence in miR319a for LUC or PHYB target sequences is indicated with green lines

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Plant Methods

ISSN: 1746-4811