Development of a seedling inoculation technique for rapid evaluation of soybean for resistance to Phomopsis longicolla under controlled conditions

Table 1 Analysis of variance of experiments for evaluating seedling cut-stem inoculation method and soybean genotypes for resistance to Phomopsis seed decay based on the area under disease progress curve (AUDPC) values for stem lesion length

Experiment	Treatment effects	Num DF^a	Den DF^b	F value	P ≥ F
1	Plant age at inoculation	3	41	3.30	0.0296
2	Fungal age at inoculation	5	6	3.19	0.0969
3	Temperature	1	3	0.94	0.4039
4	Isolate	25	16	12.80	< 0.0001
5	Soybean genotype MG III	13	13	4.69	0.0045
6	Soybean genotype MG IV	13	13	5.78	0.0017
7	Soybean genotype MG V	13	14	4.62	0.0038

Analysis of variance was performed using the generalized linear mixed model (PROC GLMMIX) of SAS (version 9.4, SAS Institute, Cary, NC) with random effects for Trial, Block within Trial, and Treatment × Trial
^aThe numerator degrees of freedom
^bThe denominator degrees of freedom calculated based on Kendward and Rogers approximation method [37]

ISSN: 1746-4811