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Fig. 8 | Plant Methods

Fig. 8

From: Use of a temporary immersion bioreactor system for the sustainable production of thapsigargin in shoot cultures of Thapsia garganica

Fig. 8

a–f NADI staining of T. garganica leaf and stem sections. Blue color indicates the presence of oxygenated or lipophilic compounds (e.g. terpenoids), concentrated mainly in epithelial cells, secretory ducts and in the epidermis. a, b Leaf cross-sections with staining visible in the epidermis. c upper-stem cross-section with the epidermis stained. d cross-section of the stem, just above callus tissue with staining concentrated in secretory ducts. e cross-sections of callus tissue at the base of the stems. Staining is visible in epithelial cells and secretory ducts. f transverse section of callus tissue showing staining of the epithelial cells along a secretory duct. g, h Unstained cross-section (g) and transverse section (h) of the stem to illustrate un-ruptured secretory ducts filled with resin/oil bodies c cortex, e epidermis (le lower epidermis, ue upper epidermis), ep epithelial cells, o oil bodies, sd secretory ducts, st stomata, vb vascular bundle. Scale bars represent 100 μm

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Plant Methods

ISSN: 1746-4811