Fig. 6
From: Cassava geminivirus agroclones for virus-induced gene silencing in cassava leaves and roots
Evaluation of silencing in leaves, fibrous roots, root tips and storage roots for a leaf- and root-expressed gene in wild-type cassava. . Plants infected with VIGS constructs targeting different endogenous genes were evaluated by RT-qPCR 6 months p.i. a Chl1 gene, coding for the Mg2+-chelatase (Manes.17G053100). b Lysin motif (LysM) receptor kinase gene (Manes.12G071800). Control plants were infected with the VIGS-uidA construct, targeting the uidA transgene. Bars represent means and standard deviations of expression levels calculated according to the Pfaffl method (2001) from three biological replicates displaying CMD symptoms (asterisks indicate significant differences in two-tailed t tests, p <0.05). The reference gene used for normalization is PP2A (Manes.09G039900). Fold change in gene expression levels as compared to the VIGS-uidA control samples is indicated above the bar for the biological samples displaying a significant reduction in gene expression. Infection rates are presented in Additional file 1: Table S1 D, E and G