Fig. 1
From: Cassava geminivirus agroclones for virus-induced gene silencing in cassava leaves and roots
Construction of a VIGS vector based on the African Cassava Mosaic Virus (ACMV) infectious clone ACMV-[NOg]. a The genome of this bipartite geminivirus was previously cloned into two pCambia 1300 plasmids containing either the DNA-A or DNA-B ACMV-[Nog] sub-genomes CRA: Common Region of DNA-A; AV1: coat protein gene; AV2: Protein V2 gene; AC3: Replication enhancer protein gene; AC2: Transcriptional activator protein gene; AC1: Replication associated protein gene; AC4: RNA silencing suppressor gene [24]. b The cassava VIGS vector was constructed by modifying the DNA-A sub-genome by replacing part of the AV1 gene with a 30-bp multiple cloning site (MCS) for insertion of gene targeting DNA fragments