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Table 1 Effects of medium, solidifying agent and co-incubation on Triphysaria root transformation

From: Factors affecting the efficiency of Rhizobium rhizogenes root transformation of the root parasitic plant Triphysaria versicolor and its host Arabidopsis thaliana

Medium

Solidifying agent

Co-incubation period

% of YFP calli (after 4 weeks)

% Transformation (after 8 weeks)

MS

Phytagel

1 week

36.8±5

24.4±1

2 weeks

75.9±12

70.0±10

Phyto agar

1 week

23.9±2

19.1±2

2 weeks

49.4±8

41.7±9

Hoagland

Phytagel

1 week

14.0±5

8.4±3

2 weeks

12.2±2

8.7±2

Phyto agar

1 week

8.6±1

6.2±2

2 weeks

7.6±1

4.8±2

Medium (M) Pr > F

  

< 0.0001

< 0.0001

Solidifying agent (S) Pr > F

  

< 0.0001

0.0002

Duration (D) Pr > F

  

< .00001

< 0.0001

S*D Pr > F

  

0.0495

< 0.0088

M*S Pr > F

  

0.0014

< 0.0043

M*D Pr > F

  

< 0.0001

< 0.0001

M*S*D Pr > F

  

0.0358

0.0209

  1. Each treatment consisted of five plates (each plate is a technical replicate) and the experiments were conducted three times (biological replicates). The efficiency values represent the average ± SD (n = 15)
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Plant Methods

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