Medium | Solidifying agent | Co-incubation period | % of YFP calli (after 4Â weeks) | % Transformation (after 8Â weeks) |
---|---|---|---|---|
MS | Phytagel | 1 week | 36.8±5 | 24.4±1 |
2 weeks | 75.9±12 | 70.0±10 | ||
Phyto agar | 1 week | 23.9±2 | 19.1±2 | |
2 weeks | 49.4±8 | 41.7±9 | ||
Hoagland | Phytagel | 1 week | 14.0±5 | 8.4±3 |
2 weeks | 12.2±2 | 8.7±2 | ||
Phyto agar | 1 week | 8.6±1 | 6.2±2 | |
2 weeks | 7.6±1 | 4.8±2 | ||
Medium (M) Pr > F |  |  | < 0.0001 | < 0.0001 |
Solidifying agent (S) Pr > F |  |  | < 0.0001 | 0.0002 |
Duration (D) Pr > F |  |  | < .00001 | < 0.0001 |
S*D Pr > F |  |  | 0.0495 | < 0.0088 |
M*S Pr > F |  |  | 0.0014 | < 0.0043 |
M*D Pr > F |  |  | < 0.0001 | < 0.0001 |
M*S*D Pr > F |  |  | 0.0358 | 0.0209 |