Fig. 4
From: A transient transformation system for gene characterization in upland cotton (Gossypium hirsutum)
Quantification of transgene expression levels in un-inoculated controls and EHA105-inoculated G. hirsutum plants. GhGPX1 (a) and GhGPX8 (b) transcript levels in cotyledons of control and transiently expressing seedlings was determined by qRT-PCR. Three independent sets of seedlings (35S:GhGPX1-1/2/3 and 35S:GhGPX8-1/2/3) were inoculated and a minimum of six different cotyledon sections from each seedling were used for the qRT-PCR assay. Endogenous WT level for each gene was given the arbitrary value of 1. Asterisks indicate significant differences from WT at **p < 0.01 (two tailed Students t-test)