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Table 3 Transformation efficiency of different chickpea cultivars. Seven chickpea cultivars inoculated with A. rhizogenes K599 strain harbouring binary vector pCAMBIA 1302

From: Genotype-independent Agrobacterium rhizogenes-mediated root transformation of chickpea: a rapid and efficient method for reverse genetics studies

Genotype Total number of explants inoculated Explants with antibiotic selection positive roots (%) Explants with GFP positive roots (%)**
Annigeri 50 73.50 (± 1.32)a* 61.62 (± 4.58)a
C-235 45 41.51 (± 2.11)d 35.58 (± 2.67)c
CPS 1 40 54.25 (± 3.14)c 41.65 (± 1.48)b
JG-62 49 72.14 (± 3.81)a 60.50 (± 2.63)a
K850 45 54.33 (± 0.99)c 40.57 (± 0.66)b
Vijay 40 63.33 (± 1.44)b 44.16 (± 1.44)b
WR-315 44 29.59 (± 2.94)e 23.51 (± 1.81)d
  1. Each value represents the mean of three independent experiments with standard deviation (SD). Approximately 40–50 seedlings were examined for each individual experiment
  2. Values in the same column followed by different letters showed significant difference among different transformation methods (Fisher's LSD test, P < 0.05)
  3. **Corresponds to the frequency of seedlings that give rise to both GFP fluorescence and PCR positive roots