Genotype-independent Agrobacterium rhizogenes-mediated root transformation of chickpea: a rapid and efficient method for reverse genetics studies

Table 3 Transformation efficiency of different chickpea cultivars. Seven chickpea cultivars inoculated with A. rhizogenes K599 strain harbouring binary vector pCAMBIA 1302

Genotype	Total number of explants inoculated	Explants with antibiotic selection positive roots (%)	Explants with GFP positive roots (%)^**
Annigeri	50	73.50 (± 1.32)^a*	61.62 (± 4.58)^a
C-235	45	41.51 (± 2.11)^d	35.58 (± 2.67)^c
CPS 1	40	54.25 (± 3.14)^c	41.65 (± 1.48)^b
JG-62	49	72.14 (± 3.81)^a	60.50 (± 2.63)^a
K850	45	54.33 (± 0.99)^c	40.57 (± 0.66)^b
Vijay	40	63.33 (± 1.44)^b	44.16 (± 1.44)^b
WR-315	44	29.59 (± 2.94)^e	23.51 (± 1.81)^d

Each value represents the mean of three independent experiments with standard deviation (SD). Approximately 40–50 seedlings were examined for each individual experiment
^∗Values in the same column followed by different letters showed significant difference among different transformation methods (Fisher's LSD test, P < 0.05)
^**Corresponds to the frequency of seedlings that give rise to both GFP fluorescence and PCR positive roots

ISSN: 1746-4811