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Fig. 6 | Plant Methods

Fig. 6

From: Genotype-independent Agrobacterium rhizogenes-mediated root transformation of chickpea: a rapid and efficient method for reverse genetics studies

Fig. 6

PA accumulation in transgenic roots of chickpea cultivar JG-62 expressing AtTT2:GFP. a Wild-type, b mock transformed and, c–e AtTT2-transformed roots after staining with DMACA reagent, f PCR amplification of TT2 (I) and virD (II) genes in A. rhizogens K599-p35S-TT2, untransformed wild-type (WT) and transgenic roots (T) and lane M is a 1-kb ladder (Invitrogen). Scale bars: for d 0.5 mm and for e 50 µm

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