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Table 1 Primers used for qRT-PCR normalization

From: Selection and evaluation of reference genes for qRT-PCR analysis in Euscaphis konishii Hayata based on transcriptome data

Gene abbreviation

Gene name

Primer sequence (5′–3′)

Amplicon length (bp)

Primers Tm (°C)

E (%)

R2

EkUBC

E. konishii Ubiquitin-conjugating enzyme E2-17 kDa

For: TCTGCAGGTCCTTCAATTCC

100

54.8/54.8

97.89

0.9998

  

Rev: CGCAAACCCTAGAGAGAGTAAG

    

EkF-ACP

E. konishii F-actin capping protein alpha subunit

For: CCAGTAACTCGCACCCTATTT

96

54.44/54.56

99.59

0.9994

  

Rev: TCACTGTCACTTTCCGATTCC

    

EkARP7

E. konishii Actin-related protein 7

For: CCTTCATTACCCATCTCCCATC

100

55.03/53.41

99.35

0.9878

  

Rev: CTAATGAATCCTCGTATGACTGGAT

    

EkEF2

E. konishii Elongation factor 2

For: GAGAGCGACAAGGGAATGAG

108

55.7/54.8

100.09

0.9997

  

Rev: TATTACTGATGGTGCGCTGG

    

EkACT

E. konishii Actin

For: CATTGTGAGCAACTGGGATG

125

54.01/54.21

103.21

0.9998

  

Rev: GATTAGCCTTCGGGTTGAGA

    

EkGAPDH

E. konishii Glyceraldehyde-3-phosphate dehydrogenase

For: TGGCTTTCCGTGTTCCTACT

113

56.14/57.12

101.1

0.9795

  

Rev: TCCCTCTGACTCCTCCTTGA

    

EkEEF-5A-1

E. konishii Eukaryotic elongation factor 5A-1

For: TCCGACATAGCTCCGATTCA

101

55.42/55.4

98.46

0.9991

  

Rev: GAAGAGACGGAGAGGAGAGATT

    

EkADF2

E. konishii Actin-depolymerizing factor 2

For: CCGAAGAGAATGTCCAGAAGAG

98

54.97/54.48

99.89

0.9998

  

Rev: GTCCTTTGAGCTCGCATAGAT

    

EkTUB

E. konishii β-Tubulin

For: AAAGATGAGCACCAAGGAGGT

108

56.18/55.60

98.69

0.9879

  

Rev: TCACACACGCTGGATTTCAC

    

EkPLAC8

E. konishii PLAC8 family protein isoform 2

For: GGGAATCGGAGGTAAAGATCAA

102

54/54

99.00

0.9822

  

Rev: TGGATCTGAAGAAATGGGAGAC

    

EkLPP

E. konishii Lonprotease-2-like protein

For: TTGGCCTCATCTATTGCTACTG

98

54.3/55.4

101.00

0.9931

  

Rev: GTTCTCCTGTGCCCTCTAATG

    

EkGSTU1

E. konishii Glutathione-S-transferase tau 1

For: GCCCTCATCCCAAACATACT

113

54.6/54

98.99

0.9999

  

Rev: GAGATTGTTTGCAGCGAATAGG

    

EkCAD1

E. konishii Cinnamyl alcohol dehydrogenase 1

For: GTGGGCTTTCCGTCAGTGTA

123

59.97/59.97

99.23

0.9969

  

Rev: GGTCGGAGTTGGAGCTATCG

    
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Plant Methods

ISSN: 1746-4811