Fig. 4

Immunoblot detection of PDC and ADH in pea roots. Plants were grown in sterile conditions and were harvested after 3 days of application of the treatments: untreated (control) roots; roots treated with pyruvate, imazamox or glyphosate; and roots grown under low-oxygen conditions. Each lane contains 30 µg of protein. a Protein blots for PDC and ADH. For each treatment, one representative sample is shown. The Coomassie-stained protein gel on the bottom of each blot shows the total amounts of input proteins. b Analyses of band intensity of blots are presented as the relative ratio to the control. The control is arbitrarily presented as 100%. Mean ± SE (n = 4). Different letters indicate significant differences between treatments (ANOVA, HSD Tukey/T3 Dunnet; p < 0.05). ADH alcohol dehydrogenase, C control, GLP glyphosate, IMX imazamox, LOW-OX low-oxygen conditions, PYR pyruvate, PDC pyruvate decarboxylase