Fig. 2

Different black-white-masks derived from the original composed root-slice image. A Root-slice image with green shining oil droplets. B–D Black-white masks of B: the oil droplets, C the center of the root slice, D the edge of the root-slice. The x–y-coordinates of the oil droplets and the center (black particles in B and C) and each point of the edge in D, and the size of the grey area (seen in B–D) were determined automatically by the image analysis software