Fig. 6
From: A simple and cost-effective method for screening of CRISPR/Cas9-induced homozygous/biallelic mutants
Identification of CRISPR/Cas9-induced etc2 mutants in Arabidopsis by MSBSP-PCR. Genomic DNA was amplified by PCR using the locus primers ETC2-F and ETC2-R (upper panel) and the amplification products used as template for a second PCR using he target specific primer ETC2-T and the locus primer ETC2-R (lower panel). M, marker; CK, negative control with ddH2O as template. L1 and L2, homozygous etc2 mutant plants; WT, wild type