Table 3 Used resources for the presented HPTLC method compared to a previously reported method combining HPLC and photometry [13]

Sample amount

1 g wholemeal flour

25 g grain, 1 g bran

Solvent used for extraction

25 mL

6 mL

Extraction time¹

108 min

36 min

Analysis time per sample

62 min HPLC

1 min photometry

6 min for both analyses

Costs for stationary phase per sample

4–6 €²

0.5 €

Consumed mobile phase per sample

14.694 mL in total³

12.106 mL water

1.147 mL acetonitrile

1.147 mL methanol

0.294 mL formic acid

1.944 mL in total⁴

0.833 mL ethyl acetate

0.555 mL 2-butanol

0.287 mL 1 mM trifluoroacetic acid in methanol

0.287 mL water

Consumed sample volume

2 µL for chromatography⁵

200–2000 µL for photometry⁶

14 µL for both analyses⁵

Time for data evaluation

Approximately the same for both protocols