Profiling and quantification of grain anthocyanins in purple pericarp × blue aleurone wheat crosses by high-performance thin-layer chromatography and densitometry

Table 1 Variation in the total anthocyanin content (TAC, kur-eq) of bran of anthocyanin pigmented wheat breeding lines and check varieties

Source	Seed colour¹	TAC_VS (µg g⁻¹ db)²			TAC_HPTLC (µg g⁻¹ db)³
Source	Seed colour¹	Min	Max	Mean⁴	Min	Max	Mean⁴
Lines	Ba + Pp	359.9	1289.6	780.5 a	466.8	1289.6	880.9 a
Lines	Ba	161.5	879.2	496.2 ab	295.1	679.3	496.3 b
Checks	Ba	117.6	669.0	353.8 bc	117.6	669.0	353.8 bc
Lines	Pp	124.3	502.5	219.7 bc	124.3	437.7	221.0 c
Checks	Pp	47.5	174.7	100.3 c	47.5	174.7	100.3 c

¹Ba, blue aleurone; Ba + Pp, deep purple (blue aleurone + purple pericarp); Pp, purple pericarp
²TAC in grain color classes based on visual scoring
³TAC in grain color classes based on chemometrics of the anthocyanin profile
⁴Means with different letters are significantly different at p = 0.05 (Tukey–Kramer test)

ISSN: 1746-4811