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Table 1 Composition of reaction mixes required per sample in a total volume of 5 μl

From: Comparison of three PCR-based assays for SNP genotyping in plants

 

Master Mix (μl)

Assay Mix (μl)

H2O (μl)

DNA (μl)

TaqMan

2.5

0.125

1.375 

1

KASP

2.5

0.07

2.43

rhAmp

2.65

0.25

1

1.1