Fig. 4From: Preparation and applications of guard cell protoplasts from the leaf epidermis of Solanum lycopersicumThe verification of GCPs in response to external stimuli by quantitative Real-time PCR. a, b Real-time PCR analyses of ABA-regulated guard cell–expressed genes in guard cells before and after MeJA (50 μM) treatment for various times. c Effect of transcription inhibitor (actinomycin D and cordycepin) in wound-responsive gene transcription during GCPs preparation. Results are shown as mean (n = 3) ± standard errorBack to article page