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Fig. 4 | Plant Methods

Fig. 4

From: Preparation and applications of guard cell protoplasts from the leaf epidermis of Solanum lycopersicum

Fig. 4

The verification of GCPs in response to external stimuli by quantitative Real-time PCR. a, b Real-time PCR analyses of ABA-regulated guard cell–expressed genes in guard cells before and after MeJA (50 μM) treatment for various times. c Effect of transcription inhibitor (actinomycin D and cordycepin) in wound-responsive gene transcription during GCPs preparation. Results are shown as mean (n = 3) ± standard error

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