Improved apple latent spherical virus-induced gene silencing in multiple soybean genotypes through direct inoculation of agro-infiltrated Nicotiana benthamiana extract

Table 1 Nineteen soybean genotypes silencing efficiency of GmPDS-VIGS silencing through the means of rub-inoculation method

Genotype	# of plants showing viral symptoms^a/# inoculated	# of plants showing photo-bleaching^a/# inoculated	% Efficiency^a
Jack	3/8	1/8	12.5
Wyandot	0/8	0/8	0
Williams	4/8	0/8	0
Williams 82	4/8	0/8	0
Thorne	0/8	0/8	0
PI 24350	2/8	3/8	37
NIL 2640	0/8	0/8	0
L83-570	0/8	0/8	0
OX-20	0/8	0/8	0
Conrad	4/8	0/8	0
Sloan	3/8	0/8	0
Magellan	3/8	3/8	38
PI 427106	7/7	2/7	29
PI 399073	10/16	10/16	63
PI 424354	11/15	7/15	47
PI 427105B	2/8	0/8	0
PI 567516C	12/15	9/15	60
PI 567301B	24/24	24/24	100
RIL 301	24/24	24/24	100

ALSV virus infection and GmPDS1 silencing efficiency of 19 genotypes, plant introductions, public cultivars, and germplasm by rub-inoculations. Symptoms were observed at soybean growth stage V2 approximately 21 dai with GmPDS-VIGS. Data as a summary of 2–3 replicates overtime with 4 plants in each replicate
^aThe total rating across multiple plants and replicates

ISSN: 1746-4811