Fig. 6
From: Isolation and manipulation of protoplasts from the unicellular green alga Penium margaritaceum
Confocal laser scanning microscopy (CLSM) images of Penium cells (a–f1) and protoplasts (a–f2) stained with intracellular dyes (green), merged with the autofluorescence signal emitted by the chloroplast (red). a DAPI staining of the nucleus, localized in the isthmus zone (1, arrow) and in a protoplast (2). b BCECF AM staining of the cytoplasm. c 5(6)-CFDA staining of the cytoplasm. d DiOC6(3) staining of the endoplasmic reticulum (ER). e Lyso Tracker™ Red staining of lysosomes and autophagic vacuoles. f Yeast Vacuole Membrane marker, MDY-64, staining of vacuole membranes. Scale bars: 15 µm