Fig. 4

Method comparisons for lipid extraction of sorghum leaf punches. Original data are presented in Additional file 6: Table S6. Ryu and Wang method (Fig. 1; n = 7), as the reference method, compared to single-extraction method with water (Fig. 2; n = 7) and the single-extraction method with a second extraction (n = 7). Significance was determined by one-way ANOVA with Tukey’s HSD test. Error bars indicate standard deviation. “H” indicates PI and PS were higher in the indicated method than in the Ryu and Wang method and “HH” indicates that PS was higher in the single-extraction method with a repeat extraction than in either of the other methods (adjusted p < 0.05 with p value adjusted using the false discovery rate). Abbreviations not indicated previously: TAG, triacylglycerol, NAPE, N-acyl phosphatidylethanolamine