Fig. 3

Method comparisons for lipid extraction of Arabidopsis leaves. Original data are presented in Additional file 5: Table S5. a Ryu and Wang method (Fig. 1; n = 4) is compared to single-extraction method with water (Fig. 2; n = 5). Ryu and Wang method was the reference method. Significance was determined by unpaired T test with unequal variance. There were no comparisons with adjusted p value < 0.05 with p value adjusted using the false discovery rate. b Addition of additives is compared to no additives in the single-extraction method with water (Fig. 2). The single-extraction method with water is the reference method. Significance was determined by one-way ANOVA with Tukey’s HSD test. Error bars indicate standard deviation. “L” indicates lipids whose levels were lower in the single-extraction with ammonium acetate method than in either of the other two methods (adjusted p value < 0.05). Abbreviations not indicated previously: acMGDG, acylated monogalactosyldiacylglycerol; DGDG, digalactosyldiacylglycerol; DGMG, digalactosylmonoacylglycerol; GIPC, glycosylinositolphosphoceramide; LPC, lysophosphatidylcholine; LPE, lysophosphatidylethanolamine; LPG, lysophosphatidylglycerol; MGDG, monogalactosyldiacylglycerol; PC, phosphatidylcholine; PE, phosphatidylethanolamine; PG, phosphatidylglycerol; PI, phosphatidylinositol; PS, phosphatidylserine; SQDG, sulfoquinovosyldiacylglycerol; Tr/TeGDG, tri- and tetra-galactosyldiacylglycerols