High-throughput analysis of amino acids in plant materials by single quadrupole mass spectrometry

Table 2 Retention times, correlations and sensitivity

Amino acid	Retention time (min)	Injection range (µM)	Correlation coefficient	LOD (µM)	LOQ (µM)
Alanine	5.02	0.5–40	0.9986	0.07	0.24
Arginine	3.29	0.5–40	0.9990	0.60	1.99
Glycine	3.67	0.5–40	0.9995	0.07	0.22
Histidine	2.60	0.5–40	0.9872	0.13	0.42
Hydroxyproline	2.71	0.5–40	0.9922	0.03	0.11
Isoleucine	7.35	0.5–40	0.9994	0.03	0.08
Leucine	7.60	0.5–40	0.9994	0.03	0.10
Lysine	5.60	0.5–40	0.9985	0.04	0.14
Phenylalanine	7.95	0.5–40	0.9995	0.05	0.15
Proline	5.21	0.5–40	0.9994	0.12	0.41
Serine	3.44	0.5–40	0.9995	0.20	0.66
Threonine	4.59	0.5–40	0.9994	0.07	0.22
Tyrosine	5.83	0.5–40	0.9992	0.02	0.08
Valine	6.05	0.5–40	0.9995	0.46	1.55
Aspartic acid	3.91	0.5–40	0.9989	0.11	0.36
Glutamic acid	4.29	2.5–200	0.9985	0.08	0.26
Cysteic acid	2.86	0.5–40	0.9996	0.58	1.94
Methionine sulfone	4.02	0.5–40	0.9997	0.05	0.18

All measured amino acids with their retention times, normal concentration range, correlation coefficient, limit of detection (LOD), limit of quantification (LOQ), and injection repeatability

ISSN: 1746-4811