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Fig. 6 | Plant Methods

Fig. 6

From: A new toolset for protein expression and subcellular localization studies in citrus and its application to citrus tristeza virus proteins

Fig. 6

CPCTV localizes to the cytoplasm and nucleus. a–d CLSM image of CPCTV–GFP after bombardment onto epidermal cells of transgenic Duncan grapefruit expressing Histone2B-RFP. CPCTV–GFP (green, a), Histone2B (red, b), brightfield image (c) and the corresponding superimposed image (d), are shown, e–k CLSM image of two cells showing BiFC YFP fluorescence after bombardment of CPCTV-nEYFP and CPCTV-cEYFP into epidermal cells of C-mac leaves. YFP fluorescence (e, i yellow), chlorophyll channel (f, blue), brightfield image (g, j) and superimposed image (h, k), are shown

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Plant Methods

ISSN: 1746-4811