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Fig. 9 | Plant Methods

Fig. 9

From: An efficient transient expression system for gene function analysis in rose

Fig. 9

RoKSN and RoFD physically interact. Representatives of spilt luciferase complementation assays in rose shoots displayed by (a, c) bright-field, and (b, d) dark-field of leaves expressing 35S:RoKSN/35S:RoFT/35S:RoCOL5 fused to amino (N)-, and 35S:RoFD/35S:RoCO fused to carboxy (C)-terminal fragment of luciferase. RoKSN and RoFD physically interact with each another (b), interactions of RoCO with RoFT and RoCOL5 were used as negative and positive control (c). This experiment was performed with five biological replicates each with three technical repeats, 20 shoots were used in each technical repeat

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