Fig. 4
From: An efficient transient expression system for gene function analysis in rose
The effect of cold treatment on transient expression efficiency in roses. 20-d old shoots were infiltrated with Agrobacterium carrying pKSN:LUC suspended with MS medium, and kept in 4 °C for 3d before LUC analysis. a Bright-field, b dark-field, c intensity of LUC bioluminescence quantified using Andor Solis image analysis software. Data are mean ± SEM of five biological replicates each with three technical repeats, 20 shoots were used in each technical repeat. Asterisks denote a significant P < 0.05