Fig. 1
From: An efficient transient expression system for gene function analysis in rose
The effect of shoot age on the transient expression efficiency of roses. The fresh cut adventitious buds were transferred on proliferation medium and cultured for 3 weeks, and then transferred to rooting medium and grown for another 3 weeks to develop roots, the seedlings with roots were then planted in soil. Sub-culture times indicated in the figure refer to the days from the first transfer until LUC analysis. a Bright-field, b dark-field, c intensity of LUC bioluminescence quantified using Andor Solis image analysis software. Data are mean ± SEM of five biological replicates each with three technical repeats, 20 shoots were used in each technical repeat. Asterisks denote a significant P < 0.05