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Fig. 4 | Plant Methods

Fig. 4

From: Evaluation of parameters affecting switchgrass tissue culture: toward a consolidated procedure for Agrobacterium-mediated transformation of switchgrass (Panicum virgatum)

Fig. 4

Overall tissue culture protocol for wild type switchgrass regeneration. a The sterilized seeds were transferred from flasks to Petri dishes. b Sterilized seeds were arranged on CIM for callus induction with 20–25 seeds per dish. c Callus formation after 6 weeks of callus induction. d Plant regeneration on REG under light intensity of 100 µmol m−2 s−1. e Regenerated shoots were observed after 2–4 weeks on REG. f Shoots (1–1.5 cm) were excised and transferred onto RM

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Plant Methods

ISSN: 1746-4811