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Table 4 Transgene (nptII) segregation analysis of T1 plants

From: Robust genetic transformation of sorghum (Sorghum bicolor L.) using differentiating embryogenic callus induced from immature embryos

Line ID

Total plants analysed

T1 segregation (positives:negatives)

Chi square value

Copy number by dPCR in T0 plants

Tx197-4

24

17:7

0.12

2

Tx197-6

24

18:6

0.50

3.9

Tx197-18

24

16:8

0.03

2

Tx197-20

24

21:3

3.12*

2

Tx197-21

21

18:3

2.28*

2.6

  1. * Event with significant difference from 3:1 segregation ratio