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Table 3 Transformation efficiency of sorghum (Tx430) DEC tissues derived from immature embryos

From: Robust genetic transformation of sorghum (Sorghum bicolor L.) using differentiating embryogenic callus induced from immature embryos

Number of DEC tissues originally bombarded

Method of selection and subculture

Total number of independent transgenic events regenerated

Transformation efficiency (%)

150

Tissues split into two equal parts, totalling 300 callus pieces

140

46.6

180

Tissues cultured as it is (no split), totalling of 180 pieces

49

27.2

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Plant Methods

ISSN: 1746-4811