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Fig. 4 | Plant Methods

Fig. 4

From: A cautionary note on the use of chromosome conformation capture in plants

Fig. 4

Reciprocal chromosome conformation capture (r3C) at the SOC1 locus of Arabidopsis using the NlaIII restriction enzyme. a Schematic diagram of the SOC1 locus showing the position of all NlaIII restriction sites (red arrowheads), and the fragments tested for interaction. The pink blocks indicate the 5′ and 3′UTR and the orange blocks highlight the position of exons along the SOC1 locus. The graph represents the results of the r3C analysis on 3-week old wild type (Col-0) seedlings with fragment N as bait. Mean (± SD) for two biological replicates is plotted. b A heat map summarizing and comparing the 3C and r3C interaction profile performed with NlaIII. The cell with a cross indicates the bait. The intensity of blue colouring is an indication of the relatively interaction frequency (scale from 0-1.0)

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