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Fig. 1 | Plant Methods

Fig. 1

From: A cautionary note on the use of chromosome conformation capture in plants

Fig. 1

Chromosome conformation capture at the SOC1 locus of Arabidopsis using the FspBI restriction enzyme. In the top panel a schematic diagram of the SOC1 locus is shown indicating the positions of all FspBI restriction sites (red arrowheads), and the fragments tested for interaction with a region spanning the Transcriptional Start Site (TSS; Fragment VII). The pink blocks indicate the 5′ and 3′UTR and the orange blocks highlight the position of exons along the SOC1 locus. The graph shows the observed relative interaction frequency of regions with the anchor VII, performed on 3 week-old wild type (Col-0) seedlings. Relative interaction frequencies are plotted on the y-axis. Distances in base pairs (bp) relative to the TSS of SOC1 are plotted along the x-axis. Mean (± SD) derived from three independent biological samples is indicated. High interaction frequencies with the bait fragment indicate potential chromatin interactions

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