Fig. 3
Validation of projected rosette area estimates. a Average projected rosette area (PRA) estimates obtained from seven individual plants (15 DAG). VIS and NIR images were analysed using iDIEL Plant (as described in the “Methods” section) or ImageJ (manual measurement). The rosettes were then detached and individual leaves were scanned and measured manually to obtain a ground truth measurement. No significant differences in PRA were observed (one-way ANOVA; p ≤ 0.05). The mean absolute percentage error (MAPE) was calculated for each dataset in relation to ground truth. b, c Validation using the Arabidopsis image dataset provided by Cruz et al. [34]. Segmentation masks for seven WT plants (plant 1, 3, 5, 7, 8, 10 and 15) [34] from VIS and NIR images at 9, 12,16 and 20 h for 9 days (15–24 DAG) are given in total number of pixels (kilopixels, Kpx) (grey). PRA estimates from respective raw VIS (yellow) and NIR (green) images analysed by iDIEL Plant are shown. Bars represent the mean ± SE. No significant differences were observed at any time point (paired Student’s t test; p ≤ 0.05)