Table 2 Ranking of candidate reference genes based on stability values calculated by four softwares for three treatments
From: Selection and validation of reference genes for RT-qPCR analysis in potato under abiotic stress
Approach | Gene | geNorm (M1) | Normfinder (M2) | BestKeeper (CV ± SD) | RefFinder |
|---|---|---|---|---|---|
Drought stress | EF1α | 0.375 | 0.079 | 3.93 ± 0.91 | 1.97 |
CUL3A | 0.772 | 0.520 | 6.93 ± 1.47 | 8.00 | |
GAPDH | 0.417 | 0.105 | 4.79 ± 1.06 | 3.83 | |
sec3 | 0.395 | 0.130 | 3.49 ± 0.75 | 2.06 | |
tubulin | 0.505 | 0.248 | 4.63 ± 1.17 | 6.19 | |
L8 | 0.432 | 0.151 | 3.30 ± 0.85 | 4.00 | |
APRT | 0.580 | 0.376 | 1.96 ± 0.52 | 3.96 | |
actin | 0.464 | 0.253 | 2.71 ± 0.67 | 2.78 | |
Osmotic stress | EF1α | 1.173 | 0.333 | 4.00 ± 0.91 | 2.06 |
CUL3A | 2.248 | 1.460 | 8.34 ± 1.89 | 8.00 | |
GAPDH | 1.829 | 1.134 | 8.00 ± 1.77 | 6.48 | |
sec3 | 1.144 | 0.206 | 2.59 ± 0.55 | 1.57 | |
tubulin | 1.482 | 0.773 | 4.43 ± 0.93 | 4.73 | |
L8 | 1.278 | 0.466 | 5.48 ± 1.17 | 2.99 | |
APRT | 1.908 | 1.105 | 7.49 ± 1.64 | 6.48 | |
actin | 1.226 | 0.129 | 3.69 ± 0.80 | 2.21 | |
Simulated drought | EF1α | 0.429 | 0.178 | 2.44 ± 0.58 | 1.86 |
CUL3A | 0.618 | 0.379 | 4.85 ± 1.10 | 8.00 | |
GAPDH | 0.515 | 0.264 | 3.90 ± 0.98 | 5.73 | |
sec3 | 0.453 | 0.233 | 2.21 ± 0.58 | 2.06 | |
tubulin | 0.562 | 0.337 | 1.62 ± 0.39 | 3.74 | |
L8 | 0.471 | 0.265 | 2.12 ± 0.53 | 2.63 | |
APRT | 0.507 | 0.262 | 4.44 ± 1.02 | 5.60 | |
actin | 0.468 | 0.214 | 4.03 ± 0.96 | 3.50 |