Skip to main content

Advertisement

Table 2 Ranking of candidate reference genes based on stability values calculated by four softwares for three treatments

From: Selection and validation of reference genes for RT-qPCR analysis in potato under abiotic stress

Approach Gene geNorm (M1) Normfinder (M2) BestKeeper (CV ± SD) RefFinder
Drought stress EF1α 0.375 0.079 3.93 ± 0.91 1.97
  CUL3A 0.772 0.520 6.93 ± 1.47 8.00
  GAPDH 0.417 0.105 4.79 ± 1.06 3.83
  sec3 0.395 0.130 3.49 ± 0.75 2.06
  tubulin 0.505 0.248 4.63 ± 1.17 6.19
  L8 0.432 0.151 3.30 ± 0.85 4.00
  APRT 0.580 0.376 1.96 ± 0.52 3.96
  actin 0.464 0.253 2.71 ± 0.67 2.78
Osmotic stress EF1α 1.173 0.333 4.00 ± 0.91 2.06
  CUL3A 2.248 1.460 8.34 ± 1.89 8.00
  GAPDH 1.829 1.134 8.00 ± 1.77 6.48
  sec3 1.144 0.206 2.59 ± 0.55 1.57
  tubulin 1.482 0.773 4.43 ± 0.93 4.73
  L8 1.278 0.466 5.48 ± 1.17 2.99
  APRT 1.908 1.105 7.49 ± 1.64 6.48
  actin 1.226 0.129 3.69 ± 0.80 2.21
Simulated drought EF1α 0.429 0.178 2.44 ± 0.58 1.86
  CUL3A 0.618 0.379 4.85 ± 1.10 8.00
  GAPDH 0.515 0.264 3.90 ± 0.98 5.73
  sec3 0.453 0.233 2.21 ± 0.58 2.06
  tubulin 0.562 0.337 1.62 ± 0.39 3.74
  L8 0.471 0.265 2.12 ± 0.53 2.63
  APRT 0.507 0.262 4.44 ± 1.02 5.60
  actin 0.468 0.214 4.03 ± 0.96 3.50