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Fig. 3 | Plant Methods

Fig. 3

From: A sensitive synthetic reporter for visualizing cytokinin signaling output in rice

Fig. 3

TCSn::GUS activity in response to exogenous cytokinins. Analysis was performed on transgenic lines germinated and grown hydroponically for 7 days followed by application of exogenous cytokinins. a, b Staining (a) and quantification (b) of GUS expression in response to application of 100 nM 6-benzylaminopurine (6-BA) for 12 h; c, d staining (c) and quantification (d) of GUS expression according to 6-BA concentration; e, f staining (e) and quantification (f) of GUS expression in response to treatment with 100 nM cytokinin fractions (Z, zeatin; ZR, zeatin riboside; KT, kinetin; iP, N6-(Δ2-isopentenyl) adenine; iPA, iso-pentenyl adenosine). Values are mean ± SD of four biological replicates. Bars = 2 cm. *P < 0.05 (ANOVA) between the control and the indicated treatments

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