Fig. 3
From: Non-destructive, high-content analysis of wheat grain traits using X-ray micro computed tomography
Image segmentation. a µCT cross section image of a typical spike in 16-bit greyscale, b initial thresholding using our adaptive method, c erosion to remove outlying objects using a disk structuring element (SE), d median filter applied to smooth and further segment region of interest (ROI), e image dilated by same SE as (c) and with remaining holder removed and f final result of this process was obtained by using (e) as a mask in conjunction with (a)